HALO® BIOCLASS肽色谱柱及各类固定相是为解决复杂的多肽分析而专门设计的，具有选择性高、稳定性好和重现性强的特点。
具有高峰容量特性的HALO®产品除了非常适用于快速、高分辨的UHPLC分析外，还兼具高温及低pH值下非常稳定的特点。此外，优化的160Å孔径非常适用于蛋白质片段分析以及肽表征等等。
HALO® BIOCLASS肽分析柱之特色:
- 以优化的160Å孔径填料所开发的一系列固定相，是多肽分析的理想选择
- 胜任由小分子量肽到高达20 kDa的多肽的超快速、超高分辨的分离
- 2µm的HALO® BIOCLASS肽分析柱与亚2µm全孔型类似柱相较，前者的峰容量要高约20%，而柱压却要低。
- 高温和低pH值下极其稳定
- 对于胰蛋白酶消化后的复杂样品, 可以将2.7µm或者5µm色谱柱进行串联使用，以达到高峰容量的要求
Applications
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Tryptic digests
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Post-Translational Modifications (PTMs)
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Variants
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Polypeptides
Features
- Fast separations
- High peak capacity
- Rugged, reliable performance
- Alternative selectivity with ES-C18, ES-CN, Phenyl-Hexyl
Available Particles
ULTRA FAST PEPTIDE SEPARATIONS
Due to the Fused-Core® design and excellent mass transferability, ultra fast separations are achievable with HALO® HPLC columns for peptide separation.
BEST USES:
Optimal particle size for LC, UHPLC, and LCMS separations of peptides.
BONDED PHASES:
MAINTAIN HIGH RESOLUTION
HALO® HPLC columns for peptide separation can be increased 2-fold while maintaining high-resolution due to the Fused Core® particle design.
POWERFUL MULTIPLEXING
In this example, two 0.2 x 250 mm HALO® 160 Å ES-C18, 5 µm nanoscale columns were connected in series to provide additional resolving power for LC analysis of the tryptic digest of reduced and alkylated trastuzumab.
Featured Documents
Application Notes
Technical Literature
Catalog
Guidebook
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